| 多肽名称 | Caspase 3 (Apopain) Substrate 1, chromogenic |
| 纯度 | HPLC ≥ 95% |
| 描述 | pNA（4-硝基苯胺）衍生的caspase底物广泛应用于各种caspase活性的比色检测。半胱天冬氨酸肽被半胱氨酸蛋白酶裂解后生成的pNA可在405nm处进行比色监测。pNA的最大吸收波长约为408nm。 Km=18μM且kcat=0.5 M-1s-1的Caspase-1底物；Km=11um、kcat=2.4m-1s-1的Caspase-1底物；Km=32um、kcat=0.05m-1s-1的Caspase-6底物；Km=180um、kcat=0.6m-1s-1的Caspase-7底物；Km=12um、kcat=0.4m-1s-1的Caspase-7底物；Km=167um的Caspase-8底物 |
| 存储 | -20°C |
| 参考论文 | Grutter MG (2000). Caspases: key players in programmed cell death. Curr Opin Struct Biol 10, 649-55; Gastman BR (2001). Apoptosis and its clinical impact. Head Neck 23, 409-25; Grutter MG (2000). Caspases: key players in programmed cell death. Curr Opin Struct Biol 10, 649-55; Stennicke HR and Salvesen GS (1999). Catalytic properties of the caspases. Cell Death Differ 6, 1054-9; Stennicke HR and Salvesen GS (1998). Properties of the caspases. Biochim Biophys Acta 1387, 17-31; Thornberry NA and Lazebnik Y (1998). Caspases: enemies within. Science 281, 1312-6; Talanian RV, et al. (1997). Substrate specificities of caspase family proteases. J Biol Chem 272, 9677-82; Fassy F, et al. (1998). Enzymatic activity of two caspases related to interleukin-1beta-converting enzyme. Eur J Biochem 253, 76-83; Datta R, et al. (1996). Activation of the CPP32 protease in apoptosis induced by 1-?-D-arabinofuranosylcytosine and other DNA-damaging agents. Blood 88, 1936-43; Koeplinger KA, et al. (2000). Caspase 8: an efficient method for large-scale autoactivation of recombinant procaspase 8 by matrix adsorption and characterization of the active enzyme. Protein Expr Purif 18, 378-87. |
| 分子量 | 638.5 |
| 多肽序列（单字母） | Ac-DEVD-pNA |
| 多肽序列（三字母） | Ac - Asp - Glu - Val - Asp - pNA |
Caspase 3 (Apopain) Substrate 1, chromogenic
Elisa Q.
2020-11-28T17:46:11+08:00
