多肽名称 | Caspase 3 (Apopain) Substrate 1f, fluorogenic |
纯度 | HPLC ≥ 95% |
描述 | AFC(7-氨基-4-三氟甲基香豆素)衍生的caspase底物广泛用于荧光法检测各种caspase活性。半胱天冬氨酸肽裂解产生强烈荧光的AFC,在500-510nm处荧光监测,激发370-390nm。 kcat/Km=1.3×106m-1s-1的Caspase-3底物,kcat/Km=1.3×104m-1s-1的Caspase-7底物 |
存储 | -20°C |
参考论文 | Gurtu V, et al. (1997). Fluorometric and colorimetric detection of caspase activity associated with apoptosis. Anal Biochem 251, 98-102; Martins LM, et al. (1997). Activation of multiple interleukin-1beta converting enzyme homologues in cytosol and nuclei of HL-60 cells during etoposide-induced apoptosis. J Biol Chem 272, 7421-30; Deveraux QL, et al. (1997). X-linked IAP is a direct inhibitor of cell-death proteases. Nature 388, 300-4; Xiang J, et al. (1996). BAX-induced cell death may not require interleukin 1 ?-converting enzyme-like proteases. Proc Natl Acad Sci USA 93, 14559-63. |
分子量 | 729.5 |
多肽序列(单字母) | Ac-DEVD-AFC |
多肽序列(三字母) | Ac - Asp - Glu - Val - Asp - AFC |
Caspase 3 (Apopain) Substrate 1f, fluorogenic
Elisa Q.
2020-11-28T17:48:34+08:00