| 多肽名称 | Caspase 3 (Apopain) Substrate 1m, fluorogenic |
| 纯度 | HPLC ≥ 95% |
| 描述 | AMC（7-氨基-4-甲基香豆素）衍生的caspase底物广泛用于荧光法检测各种caspase活性。半胱天冬氨酸肽裂解产生强烈荧光的AMC，在440-460nm处荧光监测，激发波长340-350nm。 Km=9.7µM的Caspase-3底物；Km=11µM的Caspase-7底物 |
| 存储 | -20°C |
| 参考论文 | Koeplinger KA, et al. (2000). Caspase 8: an efficient method for large-scale autoactivation of recombinant procaspase 8 by matrix adsorption and characterization of the active enzyme. Protein Expr Purif 18, 378-87; Garcia-Calvo M, et al. (1998). Inhibition of human caspases by peptide-based and macromolecular inhibitors. J Biol Chem 273, 32608-13; Thornberry NA, et al. (1997). A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis. J Biol Chem 272, 17907-11; Rano TA, et al. (1997). A combinatorial approach for determining protease specificities: application to interleukin-1 beta converting enzyme (ICE). Chem Biol 4, 149-55; Margolin N, et al. (1997). Substrate and inhibitor specificity of interleukin-1 ?-converting enzyme and related caspases. J Biol Chem 272, 7223-8; Mizushima N, et al. (1996). Ceramide induces apoptosis via CPP32 activation. FEBS Lett 395, 267-71; Faubion WA, et al. (1999). Toxic bile salts induce rodent hepatocyte apoptosis via direct activation of Fas. J Clin Invest 103, 137-45. |
| 分子量 | 675.5 |
| 多肽序列（单字母） | Ac-DEVD-AMC |
| 多肽序列（三字母） | Ac - Asp - Glu - Val - Asp - AMC |
Caspase 3 (Apopain) Substrate 1m, fluorogenic
Elisa Q.
2020-11-28T18:05:57+08:00
