多肽名称 | Caspase 1 (ICE) Substrate 2, chromogenic |
纯度 | HPLC ≥ 95% |
描述 | pNA(4-硝基苯胺)衍生的caspase底物广泛应用于各种caspase活性的比色检测。半胱天冬氨酸肽被半胱氨酸蛋白酶裂解后生成的pNA可在405nm处进行比色监测。pNA的最大吸收波长约为408nm。 |
存储 | -20°C |
参考论文 | Grutter MG (2000). Caspases: key players in programmed cell death. Curr Opin Struct Biol 10, 649-55; Gastman BR (2001). Apoptosis and its clinical impact. Head Neck 23, 409-25; Grutter MG (2000). Caspases: key players in programmed cell death. Curr Opin Struct Biol 10, 649-55; Stennicke HR and Salvesen GS (1999). Catalytic properties of the caspases. Cell Death Differ 6, 1054-9; Stennicke HR and Salvesen GS (1998). Properties of the caspases. Biochim Biophys Acta 1387, 17-31; Thornberry NA and Lazebnik Y (1998). Caspases: enemies within. Science 281, 1312-6; Talanian RV, et al. (1997). Substrate specificities of caspase family proteases. J Biol Chem 272, 9677-82; Fassy F, et al. (1998). Enzymatic activity of two caspases related to interleukin-1beta-converting enzyme. Eur J Biochem 253, 76-83; Datta R, et al. (1996). Activation of the CPP32 protease in apoptosis induced by 1-ß-D-arabinofuranosylcytosine and other DNA-damaging agents. Blood 88, 1936-43; Koeplinger KA, et al. (2000). Caspase 8: an efficient method for large-scale autoactivation of recombinant procaspase 8 by matrix adsorption and characterization of the active enzyme. Protein Expr Purif 18, 378-87. |
分子量 | 628.6 |
多肽序列(单字母) | Ac-YVAD-pNA |
多肽序列(三字母) | Ac - Tyr - Val - Ala - Asp - pNA |
Caspase 1 (ICE) Substrate 2, chromogenic
Elisa Q.
2020-11-27T21:53:14+08:00